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Fig. 3. Expression profile of the medullary zone and the PSC. (A-E) Marker
expression in the medullary zone. Medullary zone cells are characterized by
their expression of DE-cadherin (A, red), dome-gal4/UAS-GFP (B,
green), upd3-gal4/UAS-GFP (C, green), the uncharacterized GFP fusion
line ZCL2897 (D, green), and act5C-GFP (E, green). The
cortical zone is marked by Lz staining (A, green; C, red) as well as by
P1-antigen staining (E, red). (F-J) Markers expressed in the posterior
signaling center (PSC) cells. PSC cells are marked by Ser-lacZ (F-I,
red) and show high levels of expression of Dorothy-gal4/UAS-GFP (F,
green), upd3-gal4/UAS-GFP (G, green), uncharacterized GFP-trap lines
ZCL2375 (H, green), ZCL2856 (I, green) and ZCL0611
(J, green). In H and I, insets show only the GFP expression in the PSC regions
indicated by the arrows. upd3-gal4/UAS-GFP (G) is expressed in only a
subset of PSC cells. PC, pericardial cell. (K-O) Lack of expression of
specific markers also defines the PSC. PSC cells are positively marked by
Ser-lacZ (red) and characterized by absence of expression of Pvr (K,
green; inset shows the PSC region without Ser-lacZ for clarity),
dome-gal4/UAS-GFP (L, green), Cg-gal4/UAS-GFP (M, green),
U-shaped (N, green) and Hemese (O, green). To-pro-3 (blue) marks nuclei.
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