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Fig. 1. Gross phenotype of headshrinker mutants. (A-H) External views
(A,B) and skeletal specimens (C-H) of wild-type (A,C,E,G) and
headshrinker (hsk) mutant (B,D,F,H) P0 neonates. Higher
magnification views of the head region of C and D (C',D'), sternum
(E,F) and vertebral bones (G,H). Arrowheads and asterisks in E,F indicate
asymmetrical attachment of ribs to the sternum, and anteroposterior
dislocation of the sternum, respectively; arrowheads in H indicate bifurcation
and/or fusion of ribs. (I-P) The external morphologies of wild-type (I-L) and
hsk mutant embryos (M-P). The arrowhead in N indicates the
abnormality in the anterior neural folds. (Q) Phenotypic variability of
hsk mutants. Sections of wild-type (R) and hsk mutant (S)
embryos; asterisks indicate somites. c1, atlas; e, exoccipital bone; f,
frontal bone; i, interparietal bone; mn, mandible; mx, maxilla; n, nasal bone;
nt, neural tube; p, parietal bone; pm, premaxilla; s, supraoccipital bone.
Scale bar: 5 mm in C,D; 1 mm in C',D'; and 20 µm in R,S.
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