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Fig. 3. Rescue of the Ssdp1^hsk/hsk mutant phenotype by the Ssdp1 transgene. (A) Structure of the Ssdp1 transgene (TG). (B-D) Lateral view of E9.5 embryos obtained by the cross of TG#37/Ssdp1^+/hsk or TG#141/Ssdp1^+/hsk with Ssdp1^+/hsk mice. (B) Ssdp1^hsk/hsk embryo. (C) Completely rescued Ssdp1^hsk/hsk embryo carrying TG#37. (D) Partially rescued Ssdp1^hsk/hsk embryo carrying TG#141. Scale bar: 400 µm for B-D. (E) TG#37 completely rescues the mutant phenotype. (F) The expression level of Ssdp1 in two TG lines. Values shown represent the means and standard errors of the relative expression levels of the sum of endogenous and transgenic Ssdp1 RNA, as quantified by RT-PCR. The number on each bar indicates the number of samples analyzed.

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