|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Ssdp1 acts as a coactivator component of a Lim1-Ldb1-Ssdp1 complex. (A)
E8.0 Lim1-lacZ knock-in embryo stained for ß-galactosidase
activity. (B) Cross-section of the embryo shown in A; approximate sectioning
position is indicated in A. (C) Whole-mount in situ hybridization of Ldb1 in
E8.0 embryo. (D) Cross-section of embryo shown in C. Scale bars in C,D: 200
µm. (E) Schematic representation of the effector and the reporter plasmid
used in the transfection assay described in F. (F) The fusion protein
comprised of the GAL4 DNA-binding domain and Ssdp1 activated reporter gene
expression in a dose-dependent manner. (G) Schematic representation of the
effectors and a reporter used in the transfection assays described in H and I.
(H) Effects of Lim1, Ldb1 and Ssdp1 on the Gsc promoter. (I) Effects
of varying Ssdp1 concentration on the activity of Lim1- and Ldb1-mediated
reporter gene expression. Values are the means and standard errors of
duplicate experiments. (J) A model for the action of Ssdp1. Ssdp1, Ldb1 and
Lim1 constitute a ternary complex and regulate genes expressed in the
prechordal plate.
|
