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Fig. 1. slmb shows a dynamic expression pattern during egg chamber
development. Ovaries from females carrying the slmb00295
enhancer trap were analyzed by X-gal staining or by anti-ß-galactosidase
(anti-ß-gal) immunofluorescence. (A) In the ovariole, nurse cells stain
positive for X-gal throughout oogenesis (arrows). X-gal signal in FC
surrounding the oocyte appears at mid-oogenesis (arrowhead). (B,C) In the
germarium, expression of ß-gal (green) is restricted to the germline and
is excluded from FC that express Fasciclin 3 (red). (D) Expression of
ß-gal cannot be detected in FC at stage 8 (arrowhead) and starts at stage
9 (E, arrowhead). (F) At oogenesis stage 10, strong expression of the enhancer
trap occurs in a scattered pattern in the follicular epithelium (arrows). (G)
At stage 11, expression becomes stronger in two patches of FC that will form
the dorsal appendages (arrows).
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