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Fig. 4. Delayed FC differentiation and ectopic polar cells occur in slmb
somatic clones. (A) In wild-type ovarioles, Fas3 expression (red) refines as
oogenesis progresses and from stage 4 onwards becomes restricted to polar
cells (arrows). (B) In slmb FC clones, Fas3 remains widespread (egg
chambers are outlined). (C) In egg chambers bearing slmb FC clones,
large patches of cells expressing Fas3 are still detected at stage 9 (arrow).
Mutant clones from the egg chamber shown in C were positively marked with GFP
(D, arrow) and the merged confocal image shows that ectopic Fas3 correlates
with the position of the clones (E, arrow). Arrowheads in C-E mark a pair of
cells that are probably ectopic polar cells. (F-H,J) The PZ80 enhancer trap is
specifically expressed in polar cells. In wild-type ovaries at stage 8, the
two pairs of polar cells are localized at the anterior and posterior termini
of the follicle (F, arrows) at stage 8; at stage 9, anterior polar cells
migrate posteriorly between nurse cells (G, arrow); and at stage 10, they have
reached the anterior border of the oocyte (H, arrow). (I,K) The
slbo-LacZ element is expressed in border cells. In stage 10 wild-type
follicles, border cells can be seen at the anterior end of the oocyte (I,
arrow). Egg chambers bearing slmb FC clones often exhibit extra polar
cells (J, arrowhead) and differentiate ectopic clusters of border cells (K,
arrowhead). In these follicles, border cell migration is often delayed and by
stage 10, they are located between nurse cells (J,K, arrows).
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