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Fig. 6. Delay of the ath5 wave caused by loss of shh signaling.
Timecourse of ath5 expression in wild-type or mutant embryos derived
from a syut4/+ intercross. (A-D) In wild type, the wave
front is: in the ventronasal retina at 30 hpf (A); in the central retina at 35
hpf (B); and in the temporal retina at 41 hpf (C). At 50 hpf (D), the wave is
over and ath5 expression is seen only in the secondary retinal growth
zone, the ciliary marginal zone (arrows in D). (E-H) In syu mutants,
wave initiation is normal (E) but subsequently becomes delayed. About half of
mutants show normal spread of ath5 wave to central retina by 35 hpf
(F); the other half fail to show expression in central retina by 35 hpf (not
pictured; see Fig. 7B). By 41
hpf (G) the delay phenotype is fully penetrant – note absence of
ath5 expression in temporal retina at 41 hpf (arrow in G). By 50 hpf
(H), ath5 expression reaches the temporal retina and has cleared from
the central retina, much like in wild type at 41 hpf (arrows in H,C).
Nasal/anterior is left and dorsal is up in all panels. Asterisks mark the
choroid fissure. Scale bar: 50 µm. CMZ, ciliary marginal zone.
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