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Fig. 8. Effects of cyclopamine treatment on Hedgehog pathway activity and
ath5 expression. (A,B) Expression of patched1
(ptc1), a target of the Hh signaling pathway, reveals tissues in
which the Hh pathway is active. Treatment from 25-41 hpf with cyclopamine (B),
but not vehicle (A), drastically reduced or abolished the ptc1 signal
(blue stain), indicating effective blockade of Hh signaling. Expression of
patched2 was similarly affected (not shown). (C-E) Blockade of Hh
signaling at 13 hpf, but not 25 hpf, delays the ath5 wave. Larvae
treated with cyclopamine from 13-50 hpf (D) show ath5 expression by
temporal retinoblasts (arrowheads), much like a 41 hpf wild type
(Fig. 6C) or 50 hpf
syu mutant (Fig. 6H).
In vehicle-treated larvae (C), as well as larvae treated with cyclopamine from
25-50 hpf (E), the wave is over and ath5 expression is confined to
the ciliary marginal zone (arrows). Anterior/nasal is left and dorsal up in
all photos. CMZ, ciliary marginal zone; cycl., cyclopamine; veh., vehicle.
Scale bar: 25 µm for C-E.
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