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Fig. 2. Boundaries of hindbrain RA activity along the anteroposterior axis. All
embryos are homozygous for the RARE-lacZ RA-indicator transgene;
stages are indicated on each panel. Anterior is oriented towards the right in
A-D (lateral views) and towards the top in E-O (dorsal views). (A-C) Embryos
were double-stained for RARE-lacZ expression (ß-galactosidase
activity), which is observed posteriorly, followed by Cyp26a1
expression (in situ hybridization), which is observed anteriorly with its
posterior border at r2. RARE-lacZ expression is first seen at E7.5
(B) and the r2/r3 division marked in C indicates the anterior extent of
RARE-lacZ, which reaches r3 at E7.6. (D,E) Raldh2 expression
detected posteriorly in the paraxial mesoderm at E7.6. (F-K) Dorsal view of
embryos double-stained for expression of RARE-lacZ and
Cyp26a1 from E7.4-E7.9. RARE-lacZ expression has reached r3
by E7.6 (H), but begins to clear from r3 by E7.9 (K). (L-N) Double-stained
embryos showing RARE-lacZ expression (white arrows indicate its
anterior extent at the r4/r5 boundary) and expression of either
Krox20 in r3 at E8.0 (L), Krox20 in both r3 and r5 at E8.25
(M) and Hoxb1 in r4 at E8.5 (N).
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