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Fig. 6. Maternal dietary RA supplementation rescues r3/r4 and r4/r5 gene expression
boundaries in Raldh2–/– embryos. Embryos were
subjected to low-dose maternal dietary RA supplementation from E6.75-E8.0
(A,F-H) or from E6.75-E8.25 (B-E). (A) RARE-lacZ expression in
RA-treated wild-type and Raldh2–/– embryos at
E8.0. The Raldh2–/– embryo exhibits an
anterior border of RARE-lacZ expression similar to that observed in a
wild-type littermate, although the intensity of staining was less than that
observed in the wild-type embryo. (B-E) RA treatment of wild-type and
Raldh2–/– embryos followed by analysis at
E8.75 demonstrates that RA administration rescues r4 expression of
Hoxb1 (B,C) and Cyp26c1 (D,E) in
Raldh2–/– embryos. RA-treated wild-type
embryos exhibited relatively normal expression of Hoxb1 and
Cyp26c1. All embryos were also simultaneously stained for
Raldh2 expression, which appeared only in wild-type embryos as
indicated. (F-H) RA treatment of Raldh2–/–
embryos followed by analysis at E8.0 results in posterior hindbrain expression
of vHnf1 (F-G) and relatively normal r4/r5 restriction of
Irx3 (H). Embryos were double stained for Raldh2 expression,
which was observed only in wild-type embryos (brackets).
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