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Fig. 5. bNCSCs show an intrinsic ability to differentiate into the sensory lineage.
Comparison between bNCSCs and NCSCs isolated from the sciatic nerve from E12.5
mouse embryos (sNCSCs) established with the same protocol as the bNCSCs. (A) A
50 ng/ml Bmp2 treatment during a 5-day differentiation leads to increased
neuronal differentiation in both types of NCSCs. Black bars are Bmp treated;
white bars are untreated cultures. (B,C) ßIII-tubulin immunocytochemistry
of NCSCs treated with 50 ng/ml of Bmp2. sNCSCs are larger in size. (D,E) Same
cells as in B and C showing lack of immunoreactivity for tyrosine hydroxylase.
(F,G) X-gal staining of cells differentiated for 5 days showing Fkh3-positive
cells in the bNCSCs, but not in the sNCSCs. (H) Quantification of experiments
in F,G (n=25 and n=45, respectively). Abbreviations: BC,
boundary cap; SN, sciatic nerve; Bmp, bone morphogenic protein. Scale bars: in
B, 25 µm for B,D; in C, 25 µm for C,E; 25 µm in F,G.
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