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Fig. 2. Anxa5-lacZ reporter is specifically expressed in the
vasculature of E10.5 embryos. Sagittal sections (A-D,F-I) of heterozygous
embryos were stained for ß-galactosidase activity (A,F) followed by
immunodetection of SMA (B,G), PECAM (C,H) and nuclear staining by DAPI (D,I).
In the overlays (E,J), X-gal deposits are indicated by white spots.
Anxa5-lacZ+ cells are restricted to
SMA+/PECAM+ dorsal aorta and PECAM+
capillaries (E). At higher magnification, only a subset of PECAM-associated
cells was positive for lacZ, as shown by DAPI staining (J, arrows).
(K-N) Anxa5-lacZ+ cells are located in regions
positive for the pericyte-specific marker NG2-proteoglycan in embryonic brain
at E13.5. Transverse sections of capillaries were stained for
ß-galactosidase activity (K, arrows). Positive areas appear black because
of the monochrome character of the camera system. In parallel, PECAM (L) and
NG2-proteoglycan (M) were detected by immunostaining. After merging,
colocalization of PECAM and NG2 appears orange (N). X-gal deposits are shown
as white spots (N, arrows). Scale bar: 50 µm.
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