|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Anxa5-lacZ+ cells differentiate into early
chondrogenic, osteogenic and adipocyte lineages in vitro. Aggregate cultures
of PVCs were cultivated in chondrogenic medium (A-F,M-O). By day 14
(B,C,E,F,M-O) or 21 (A,D), cryosections were performed and strong staining was
seen for collagen VI (Col6; C) and collagen IX (Col9; B), whereas collagen II
was barely detectable (Col2; A). Aggregate sections were stained for neutral
fat deposits using oil Red O staining (red droplets) and Hematoxylin (M) as
well as for PPAR
2 (N). Additionally, isolated PVCs were cultivated in
osteogenic medium for 21 days (G-L). Ca2+ deposits in cryosections
of these aggregates were detected by Alizarin Red (H) as well as van Kossa
staining (I). Collagen I was detected by immunostaining (G). Aggregates of
PVCs were cultivated in proliferating medium as negative control for the
staining of Ca2+ deposits (K,L). Secondary antibodies were used as
negative control for the immunostaining (D-F,J,O). Scale bars: 50 µm.
|
