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Fig. 7. Purified PVCs participate in muscle regeneration in vivo. Isolated
Anxa5-lacZ+ PVCs of adult brain meninges were injected
into regenerating areas of crushed tibialis anterior (TA) muscle of
immunodeficient NOD/SCID mice. By day 13, consecutive muscle sections were
analyzed for ß-galactosidase activity by X-gal staining and expression of
desmin (B,E), PECAM (C) as well as CD34 (F) by immunohistochemistry. Injected
Anxa5-lacZ+ cells were detected at day 13 in the
zone of regeneration (arrows, A,D) in columnar structures associated with
intact desmin-positive muscle fibers (B,E). No staining was seen in intact
fibers. lacZ-expressing cells were found in areas positive for
CD34+ (D,F) but not in areas positive for PECAM (A,C). Scale bars:
50 µm.
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