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Fig. 8. Purified Anxa5-lacZ+ PVCs do not have
hematopoietic capacity in vivo. Purified
Anxa5-lacZ+ PVCs from adult brain meninges
(Ly5.2+, 1 x105 cells) were mixed with bone marrow
cells (Ly5.1+, 9 x105) and injected intravenously
into recipient mice (Ly5.1+) after lethal irradiation. After 28
days, peripheral blood of recipients was analyzed by flow cytometry for the
presence of Ly5-markers. (A) No Ly5.2+ cells were detectable in
blood of the negative control mice, represented by non irradiated wild-type
Ly5.1+ mice without transplantation. (B) In the positive control,
18% of the blood cells derived from the injected Ly5.2+ BMCs. (C)
No Ly5.2+ cells could be detected after administration of
Anxa5-lacZ+/Ly5.2+ donor PVCs.