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Fig. 8. Purified Anxa5-lacZ+ PVCs do not have hematopoietic capacity in vivo. Purified Anxa5-lacZ+ PVCs from adult brain meninges (Ly5.2+, 1 x105 cells) were mixed with bone marrow cells (Ly5.1+, 9 x105) and injected intravenously into recipient mice (Ly5.1+) after lethal irradiation. After 28 days, peripheral blood of recipients was analyzed by flow cytometry for the presence of Ly5-markers. (A) No Ly5.2+ cells were detectable in blood of the negative control mice, represented by non irradiated wild-type Ly5.1+ mice without transplantation. (B) In the positive control, 18% of the blood cells derived from the injected Ly5.2+ BMCs. (C) No Ly5.2+ cells could be detected after administration of Anxa5-lacZ+/Ly5.2+ donor PVCs.





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