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Fig. 1. Downregulation of cardiac Bop expression in Mef2c mutant
embryos. (A) Southern blot of PCR-amplified cDNA derived from hearts of
wild-type and Mef2c mutant embryos at E9.0 demonstrated a decrease of
Bop expression in hearts from Mef2c mutant embryos. The
-enolase transcript, which is expressed ubiquitously and is independent
of Mef2c expression, was used as an internal control. (B)
Differential Bop expression is detected by in situ hybridization in
sections of wild-type and Mef2c mutant embryos at E9.0. Bop
transcripts are significantly downregulated in the Mef2c mutant. a,
atrium; lv, left ventricle; rv, right ventricle; v, ventricle.
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