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Fig. 5. Chamber formation in Tbx20 mutant hearts. Whole-mount in situ
hybridization analysis of expression of cardiac chamber markers in wild-type
and Tbx20–/– hearts at E9.5. All views are
from the left lateral side, anterior up. Expression of markers for ventricular
(arrows in wild-type embryos in A-F) and for atrial chamber myocardium
(arrowheads in A-C) are missing in the corresponding stage-matched
Tbx20 mutant hearts. Chisel is expressed very weakly in the
primitive ventricle of the Tbx20–/– heart
(arrow in B'). Vessel formation is affected in the mutant, as shown by
Cx40 expression, which marks branchial arch vessels and dorsal aorta
in the wild type (white arrows in C), but reveals only scattered cells in the
branchial arch region in the mutant (white arrow in C'). Tbx3
expression is only found in the atrioventricular canal of the wild-type heart
(arrow in G). (H,H') Tbx18 expression in the proepicardial
organ (arrow) and the septum transversum (arrowhead) is unchanged in
Tbx20–/– embryos. Note that expression of
markers for pharyngeal mesoderm, Hey2 and Tbx3 (arrowheads
in D and G) is preserved in Tbx20 mutants (arrowheads in D' and
G'). Markers and genotypes are indicated in the figure.
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