|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Analysis of primary myocardium in Tbx20–/–
hearts. Whole-mount in situ hybridization analysis in E7.5 to E9.5 embryos
shows the presence of markers for primary myocardium (Tbx2, Bmp2) and
endocardium (vinexin 
) in the heart tube of Tbx20 mutant
embryos. (G-H') Anti-PECAM immunohistochemistry for endocardial
endothelium. Images are of whole embryos (A,A') and magnifications of
anterior body regions, including the heart (B-N'), in views from the
right lateral side (A-B',D,D',F-I',K-L',N,N'),
or from the ventral side (C,C',E,E',J,J',M,M').
Anterior is up. Embryos are E7.5 (A,A'), E8.25 (B-C'), E8.5
(D-E',G,G',I-J',L-M') and E9.5
(F,F',H,H',K,K',N,N'). Arrow in A' marks
Tbx2 expression in the cardiac crescent in Tbx20 mutant
embryos. Arrows in all other figures point to the left ventricle that is free
of hybridization signals for markers of primary cardiac phenotype. By
contrast, corresponding mutant hearts show expression of these genes in the
primitive ventricle (arrowheads in lateral views of E8.5 and 9.5 mutant
hearts). Note the increase of Tbx2 expression (C') but decrease
of Bmp2 expression (M') in the primitive inflow tract of the
E8.5 mutant heart. In wild-type embryos, Tbx2 expression is
restricted to the atrioventricular canal (avc) and the septum transversum
(st). al, allantois. Markers and genotypes are indicated in the figure.
|
