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Fig. 6. Distinct functions for Mash1 and Ngn2 in specification of dorsal neurons. Immunofluorescence on cross-sections of E11.5 mouse neural tubes where Mash1 and Ngn2 have been swapped into the Ngn2 and Mash1 locus, respectively: wild type (A,F,K), Mash1^{KI Ngn2/+} (B,G,L), Mash1^{KI Ngn2} (C,H,M), Ngn2^{KI Mash1/+} (D,I,N) and Ngn2^{KI Mash1}(E,J,O). (A-E) Co-localization of Brn3a (red) and Lhx1/5 (green) detects dI2 neurons (yellow). (F-J) Isl1 (green) detects dI3 neurons. (K-O) Pax2 (green) and Lmx1b (red) detect dI4 and dI5 neurons, respectively. Cell counts for each marker on at least three sections from at least three embryos of each genotype are shown in the table. (P) Because dI4 and dI6 populations cannot be distinguished in the Mash1 null, all Pax2 counts were completed by drawing a boundary line at the first Pax2-expressing cell found nearest the ventricular zone, and as such, they are labeled as dI4/6. ^**P<0.001 and ^*P<0.01. Scale bar: 50 µm.

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