|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. RA increases SVZ cell proliferation in explants. (A,B) Bis-benzimide
staining of P2 slices cultured for 2 DIV with vehicle (Veh, A) or 10 µM RA
(B) and re-sectioned at 40 µm. The SVZ and RMS stain as a dense band that
expands after RA treatment. (C-F) BrdU immunostaining of the sections in A and
B; slices were exposed to BrdU for 2 hours prior to fixation. More SVZ cells
incorporated BrdU with RA treatment (D,F) compared with control (C,E). (E,F)
Show regions of SVZ approximated by the boxed area in C. (G) Quantification of
BrdU-positive cell numbers per fixed SVZ area (boxed region in C).
**Denotes P<0.01. (H,I) TUNEL staining of control (H)
and RA-treated (I) explants. No difference in TUNEL-stained cells (arrows) was
seen (see text for quantitation). CTX: cortex. OB: olfactory bulb. Asterisk
marks lateral ventricle in A. Scale bar: in B, 100 µm for A-D and 25 µm
for E,F; in I, 50 µm for H,I.
|
