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Fig. 6. Retinol stimulates SVZ neurogenesis in explants. (A-D) BrdU immunolabeling
of SVZ cells (arrows) in slices incubated with vehicle (Veh; A), retinol (Ro;
B), disulfiram (Ds, C), or Ds and Ro (D). Explants were treated for 2 days,
exposed to BrdU for 2 hours before fixation, and then resectioned at 40 µm.
Note the increased BrdU labeling in the Ro-treated slice (B) compared with
vehicle (A) or Ds (C), and the lack of increased BrdU-positive cells in the
explant cultured with both Ro and Ds (D). E-H: Confocal images of explants
treated as in A-D and immunostained with an anti-calretinin antibody. Ro
treatment (F) expanded the RMS (arrows) compared with the other groups, and
the effect was blocked by co-treatment with Ds (H). I: Quantification of
BrdU-positive cell numbers per fixed SVZ area (see
Fig. 4C). **Denotes
P<0.01. CTX: cortex. RMS: rostral migratory stream. OB: olfactory
bulb. Scale bar: 100 µm.
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