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Fig. 4. Activity of Xema activator and repressor constructs. (A) VP-Xema-mediated
inhibition of mesoderm formation. RT-PCR analysis of animal caps dissected at
late blastula stages and cultured until midgastrula stages. VP-Xema
RNA (1 ng) was injected at early cleavage stages, as indicated. Activin (0.5
ng/ml) was added to stage 9 animal caps, as indicated. (B) Expression of
EnR-Xema induces mesoderm. RT-PCR analysis of animal caps dissected at late
blastula stages and cultured until midgastrula stages. EnR-Xema RNA
(1 ng) was injected at early cleavage stages, as indicated. (C) Expression of
EnR-Xema induces ectopic structures. Whole-mount immunohistochemistry of
tailbud stage embryos using the somite-specific antibody 12/101; lateral
views, anterior is to the right. Embryos were stained with X-Gal as a
substrate prior to immunohistochemistry. The embryo on the right was
co-injected with 100 pg lacZ and 1 ng EnR-Xema RNA in the
animal pole at the two-cell stage; note the absence of somite staining (red)
in the secondary structure containing the ß-galactosidase activity
(blue). The embryo on the left was injected with 100pg lacZ RNA in
the animal pole at the two-cell stage and was not probed with the
somite-specific antibody.
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