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Fig. 4. Wnt2b downregulates the expression of multiple proneural genes. (A-F) mRNA
expression pattern of Notch1, Cath5, NeuroM, and Delta1 in the central region
of control (A,C,E,G) or Wnt2b-expressing (B,D,F,H) E5.5 retina. These genes
were robustly expressed in the control retina, but their expressions were
reduced in the Wnt2b-expressing retina, except for Delta1, expression of which
was unaffected by exogenous Wnt2b. (I) RT-PCR analysis of proneural gene
expressions. The expression of all of the genes, Notch1, Cath5, NeuroM, Cash1,
Neurogenin 1(Ngn1), and Neurogenin 2 (Ngn2) was downregulated in the
Wnt2b-expressing retina (right lane) compared to the control retina (left
lane), whereas the expression level of Delta1 and Gapdh was unchanged. The
expression of Lef1 was upregulated in the Wnt2b-expressing retina. (J)
Percentage expression of each proneural gene in Wnt2b-expressing retina to
that in the control retina. Intensity of PCR products corresponding to each
proneural gene was measured and normalized to that of Gapdh. Relative signal
intensity of each gene in the control experiment was represented as 1. Scale
bar: 30 µm.
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