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Fig. 2. soAE is a direct target of so. soAE (sine oculis
autoregulatory element) corresponds to fragment number 21. Arrows indicate the
ocellar region. (A-C) soAE harbours three putative
transcription-factor-binding sites, which have been mutated. (A) Mutating the
core of the HD recognition sequence (mutHD) or (B) mutating the GATA sequence
(mutGATA) abolishes expression of the lacZ reporter in the ocellar
region. (C) so10-mutPAX in which the putative Pax6-binding site is
mutated, mediates expression indistinguishable from wt/so10-soAE-mediated
expression. (D-F) Oligomerization of soAE boosts its expression. By contrast
to soAE alone, which only mediates expression in the posterior margin of the
eye disc (D), 4xsoAE drives expression posterior of the MF, within the MF and
in some cells in front of the MF, but not in the ocellar region (E). This
expression resembles wt so-expression despite the ocellar region. (F)
10xsoAE does not further amplify expression intensity but results in a more
blotchy type of expression pattern. (G) Sequences of soAE and the mutated
versions of it. Sequences TAA and GAT of soAE are important for the
ocellus-specific expression of so10-soAE-lacZ. (F) Radiolabelled
probes of mutHD and mutGATA are not shifted by SO in EMSA. By contrast, mutPAX
is bound by SO and therefore shifted in EMSA.
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