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Fig. 6. hh contains functional SO-binding sites. (A) Genomic map of the
hh locus. The physical mapping is indicated as: X, XhoI; S,
SacI; K, KpnI. The hh1 (bar-3)
deletion is mapped according to Lee et al.
(Lee et al., 1992). Two
SO-binding sites are located within the region deleted in
hh1 (sequences are given in
Fig. 4C: hh first,
hh second). One additional SO-binding site is found upstream of the
hh1 deletion. (B) hh1-lacZ:
lacZ expression posterior to the MF is mediated by 1.4 kb genomic DNA
from the region deleted in hh1. (C)
hh1 SOmut: expression is hardly detectable when the two
SO-binding sites are mutated. (hh first, GAG is changed to CCC;
hh second, GAT is changed to CCC). (D) hh1
5'-lacZ: no expression is detected from a reporter
construct containing only one mutated SO-binding site
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