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Fig. 2. Reporter activity in different stages and culture length. pTP-1Venus reporter construct was transfected in stage 28 or 29 PV and cultured for 3, 24 and 48 hours. (A) After 3 hours of culture, stage 29 PV shows the reporter activity in the uninvaginated epithelium in a scattered fashion. No signals were detected in invaginating epithelial cells (arrowheads). (B) Transfected stage 28 PV were cultured for 24 hours. Gland formation is comparable with that in stage 29 PV after 3 hours of culture (A). A significant number of Venus-positive cells (arrowhead) is observed in invaginating epithelial cells. (C) Stage 29 PV were cultured for 48 hours. Scattered Venus-positive cells are still observed, and obvious gland cells show reporter activity. Scale bar: 100 µm.





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