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Fig. 8. Numb and Su(H)DBM rescued the effect of CNIC. Stage 28 PV, co-transfected with pTP-1Venus reporter construct, CNIC expression vector, and Notch inhibitor construct, were cultured for 36 hours. Both Numb and Su(H)DBM reduce excess activation of Notch signaling (compare B and D with F) and reduce well-spaced gland formation (lack of chicken SP expression in neighboring sections; compare A and C with E). More than 12 explants were investigated. More than 30 sections from each explant were investigated in three experiments. Scale bar: 100 µm.





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