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Fig. 9. Numb and Su(H)DBM transfected cells
preferentially contribute to the luminal epithelium. Efficiently transfected
Numb (I') and Su(H)DBM (E')
suppressed gland formation. Numb-transfected cells (anti-FLAG
immunostaining; G',I') and
Su(H)DBM-transfected cells (co-transfected EGFP;
C',E') selectively localize in the luminal epithelium but not the
small glands (C',G'; arrowheads). Expression of EGFP in
control explants was observed both in the luminal and glandular epithelium
(A-B). Chicken SP expression in the neighboring sections (rightmost
column) indicates the differentiation of luminal epithelium. Gland formation
was morphologically confirmed (leftmost column). More than 100 explants for
Numb and 12 explants for Su(H)DBM were
investigated. More than 30 sections from each explant were investigated in at
least three experiments. Arrowheads indicate small glands. Scale bar: 100
µm.
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