DEV01858 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Lim1-lacZ knock-in reporter reflects endogenous Lim1 expression. lacZ staining (blue) in Lim1^lz/+ mice during kidney development. (A) Posterior view of gastrula at E7.5. (B) Dorsal view at E8.5. (C) Lateral view at E9.5. (D) Cross-section at E9.5. (E) Ventral view of the anterior nephric duct at E10.5. (F) Lateral view of the posterior nephric duct at E10.5. (G) Sagittal section of the anterior nephric duct at E10.5. (H) Sagittal section of the posterior nephric duct at E10.5. (I) Dorsal view of urogenital system at E12.5. (J) Cross-section of the metanephros at E12.5. (K) Ventral view of urogenital system at E14.5. (L) Section of the metanephros at E14.5. The white arrowhead indicates an immature glomerulus. cb, comma-shaped body; cm, condensed mesenchyme; gl, glomerulus; gu, gut; k, kidney (metanephros); lm, lateral mesoderm; lpm, lateral plate mesoderm; md, Müllerian duct; mm, metanephric mesenchyme; mt, mesonephric tubule; mv, mesonephric vesicle; nd, nephric duct; nm, nephric mesenchyme; nt, neural tube; pa, pretubular aggregate; pm, posterior midline tissue; sb, S-shaped body; sm, somite; st, stalk; ub, ureteric bud; wd, Wolffian duct.

• Supplemental Figure 2 -

  Fig. S2. Urogenital organ abnormalities in adult nephric duct epithelium-specific Lim1 knockout mutant mice. Hoxb7Cre^tg/+; Lim1^flox/+ (A,C,E,G,J) and Hoxb7Cre^tg/+; Lim1^lz/flox (B,D,F,H,I,K) (A-F) Eigh-week-old males. (A,B) Ventral view of metanephros. Adult Hoxb7-Cre^tg/+; Lim1^lz/flox mice have small kidneys. (C,D) Lateral view of testis and male reproductive tract. The white arrowhead in D indicates residual tissue of the male reproductive tract. (E,F) Seminal vesicle. In Hoxb7-Cre^tg/+; Lim1^lz/flox mutant males, most of the male reproductive tract organs were absent and the lobes of the seminal vesicles were reduced. (G-K) Females at 4 weeks of age. (G-I) Female reproductive organs. Hoxb7-Cre^tg/+; Lim1^lz/flox mutant females completely or partially lacked the uterus, The arrowheads in H and I indicate absence of uteri presence of connective tissue. The anterior uterus is lost and most of the uterus is hypoplastic although the oviducts and posterior uterus are normal in I. (J,K) Genitalia region. Hoxb7-Cre^tg/+; Lim1^lz/flox mutant females had vaginal atresia (white arrowhead in K). a, adrenal gland; an, anus; ed, epididymis; ge, genitalia; k, kidney (metanephros); od, oviduct; ov, ovary; t, testis; ut, uterus; vd, vas deferens; vg, vagina. Cont, control; mut, mutant.