|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Hypomorphic phenotypes in BAC transgene-rescued Lim1 mutants. (A)
Schematic diagram of mouse BAC clones around the Lim1 locus.
(Adapted, with permission, from Celera Discovery System.) (B) Breeding scheme
to generate BAC transgene-rescued Lim1 mutants. (C) PCR genotyping of
neonates. Because two different null alleles of Lim1
(Lim1neo and Lim1lz) were used, BAC
transgene-rescued Lim1 mutants were easily identified by PCR
genotyping. (D-Q) Phenotypes of Lim1-null (D,H), BAC
transgene-rescued Lim1 mutants for line 5 (E,I,L,O) and line 7
(F,J,M,P), and wild-type (G,K,N,Q) mice at birth. (D-G) Lateral view of the
head region. Insets in D and E show frontal views. Arrowheads indicate ears.
(H-K) Ventral view of urogenital system of female neonates. Arrowheads
indicate connective tissues that lack the uterus, which is removed and not
shown in H. (L-N) Hematoxylin and Eosin-stained metanephros. (O-Q) High
magnification of the cortical region of the metanephros in L-N, respectively.
(R-Y) Whole-mount in situ hybridization with a Lim1 probe at E12.5 of
the metanephros (R-U) and urogenital ridges (V-Y) from BAC transgene-rescued
Lim1 mutants (Line 7) (R,S,V,W) and wild-type (T,U,X,Y). (S,U,W,Y)
High-magnification images in R, T, V and X. a, adrenal; cd, collecting duct;
cm, condensed mesenchyme; g, gonad; ge, genitalia; gl, glomerulus; k, kidney;
md, Müllerian duct; np, nephron progenitor; ov, ovary; tu, tubules of
nephron; ub, ureteric bud; ur, ureter; ut, uterus.
|
