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Fig. 6. Molecular expression analysis of nephric duct epithelium-specific Lim1 knockout mutants. Lim1lz/flox (A,M), Hoxb7Cretg/+; Lim1flox/+ (C,E,G,I,K,O) and Hoxb7Cretg/+; Lim1lz/flox (B,D,F,H,J,L,N,P) mice. (A-J) Lateral view of posterior nephric duct. (A,B) Lim1-lacZ expression at ts6. (C-J) Whole-mount in situ hybridization at ts9 with probes for Ret (C,D), Wnt11 (E,F), Gdnf (G,H) and Pax2 (I,J). Arrows indicate ureteric bud; arrowheads indicate metanephric mesenchyme. (K-P) Dorsal view of the metanephros at ts18 with Ret (K,L), Lim1-lacZ (M,N) and Wnt11 (O,P) expression. Arrows indicate ureteric bud tips.





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