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Fig. 2. 12D10D encodes a novel G protein-coupled receptor expressed throughout
early Xenopus development. (A) Amino acid sequence alignment of XFlop
C, full-length XFlop (FL) and Xgpcr4, showing that XFlop and Xgpcr4
have diverse cytoplasmic tails. The seven transmembrane domains are
highlighted in cyan; the putative G protein-coupling motif (DRF) is
highlighted in mauve. (B) The overall percentage sequence identity and
homology among members of GPR4 subfamily. (C) Pustell matrix analyses show
that XFlop and Xgpcr4 reside on the same chromosome (represented by scaffold
3407). The continuity of the plotting line is used to represent homology: a
solid line represents greater than 65% homology between the two sequences,
whereas areas where the line is broken indicate that the homology between the
two sequences is less than 65%. (D) The cDNA sequence identified from the
library screen contains an extra A at position 947 of the putative ORF
(highlighted in green), which causes a premature stop codon (highlighted with
red in the upper row). (E) Real-time RT-PCR data showing that XFlop is
expressed throughout early development (left), and is ubiquitously expressed
in the early to late blastulae (right).
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