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Fig. 4. ANI-1 is required for the formation of cortical patches containing myosin
II and the septins. (A) Wild-type embryos and embryos depleted of ANI-1, both
UNC-59 and UNC-61, or NMY-2 were fixed prior to pronuclear migration (when the
anterior cortex normally ruffles) and stained for ANI-1, NMY-2 and UNC-59.
Ruffles are not preserved by the fixation. In wild-type embryos, all three
proteins concentrate in an inhomogeneous network of cortical patches. In
ani-1(RNAi) embryos, UNC-59 and NMY-2 are diffuse on the cortex.
Scale bar: 10 µm. (B) Schematic showing region of embryo magnified in C.
(C) Magnified view of the cortex from control and ani-1(RNAi) embryos
in A. Scale bar: 5 µm.
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