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Fig. 5. ANI-1 depletion does not inhibit cortical or cytoplasmic flows or the establishment of polarity. (A) Kymographs of yolk granule flow generated from timelapse DIC sequences of control and ani-1(RNAi) embryos collected between meiosis II and pronuclear meeting. Red line: cytoplasmic yolk granules in the center of the embryo flow towards the posterior. Green line: cortical yolk granules in the posterior flow towards the anterior. Vertical scale bar represents 5 minutes; horizontal scale bar represents 10 µm. (B) Left: selected images from timelapse sequences of control and ani-1(RNAi) embryos expressing NMY-2:GFP (see also Movies 4 and 5 in the supplementary material). Right: kymographs showing the behavior of cortical NMY-2:GFP over time (8 minutes, 20 seconds preceding pronuclear meeting). The maximum fluorescence intensity for each point across a 2 µm wide box (broken lines) is displayed as a strip (x-axis) for each timepoint and placed sequentially to generate kymographs (time is on the y-axis). NMY-2:GFP patches (control) and smaller fluorescence discontinuities [ani-1(RNAi)] in the embryo posterior moved towards the anterior. Times are given in minutes:seconds. Scale bar: 10 µm.

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