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Fig. 9. Model for the role of ANI-1 in the formation of cortical patches. (1) ANI-1 at the plasma membrane binds F-actin. (2) F-actin bound to ANI-1 recruits additional ANI-1 to form a cortical meshwork. (3) Patches of ANI-1 and F-actin recruit myosin II. (4) Myosin II contractility produces ingressions. ani-1(RNAi): patches of cortical myosin II and ingressions fail to form, but flows are normal. nmy-2(RNAi): ANI-1-containing patches still form, but do not ingress and cortical flows fail.





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