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Fig. 2. Anillin and F-actin localization during cellularization. Indirect immunofluorescence of formaldehyde-fixed embryos using laser confocal imaging. (A,C) Wild-type, (B,D) anillin^PQ/RS-derived embryos. Scale bar: 5 µm. (A) Wild-type embryo in slow phase, sectioned perpendicular to embryo surface. The teardrop-shaped furrow canals (white arrowheads) contain high levels of Anillin and F-actin. (a) Same embryo, sectioned parallel to the surface at the cellularization front. There is an almost hexagonal network of furrow canals, with Anillin and F-actin partially colocalized in bar-like structures. (B) anillin^PQ/RS-derived embryo in slow phase, sectioned perpendicular to embryo surface. Furrow canals are malformed (white arrowhead), with lower Anillin levels than wild type. (b) Same embryo, sectioned parallel to the surface at the cellularization front. The network of furrow canals is malformed and partially disorganized, appearing fuzzy. (C) Wild-type embryo in late cellularization/early gastrulation, sectioned perpendicular to embryo surface. Ring-shaped Anillin and F-actin assemblies exist at the base of the newly formed cells (c,c'). The rings sit on top of stalks that connect the cells to the yolk mass (see Fig. S1 in the supplementary material). (D) anillin^PQ/RS-derived embryo in late cellularization/early gastrulation, sectioned perpendicular to embryo surface. The ring-shaped Anillin and F-actin assemblies are missing (d,d'), and a disorganized F-actin network containing very little Anillin is present at the base of the cells.

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