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Fig. 3. Anillin and Myosin II localization during late cellularization. Indirect immunofluorescence of heat, followed by methanol, fixed embryos using laser confocal imaging. (A) Wild type; (B) anillin^PQ/RS-derived embryos. Scale bar: 5 µm. (A) Wild-type embryo in late cellularization, sectioned perpendicular to embryo surface. The cellularization front is rich in colocalized Anillin and myosin II. Little Anillin is present in nuclei. (a-a'') Same embryo, sectioned parallel to the surface at the cellularization front. Contractile rings beneath each nucleus are rich in colocalized Anillin and Myosin II. (B) anillin^PQ/RS-derived embryo in late cellularization, sectioned perpendicular to embryo surface. The cellularization front contains less Anillin. Much of the Anillin has moved into nuclei. Some of the nuclei are mis-positioned (white arrowhead). (b-b'') Same embryo, sectioned parallel to the surface at the cellularization front. Contractile rings are absent. Instead, Anillin and part of the Myosin II are present in abnormal bars. Some nuclei are visible in this plane of section (arrowheads), indicating mis-positioning relative to the embryo surface.

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