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Fig. 8. TEM analysis of pole cells. Wild-type embryo in fast phase, showing a region near the posterior end of the embryo. PC, pole cell nucleus with characteristic round morphology with smooth nuclear envelope. CC, cellularizing cell nucleus. Stalks connecting the cells to the yolk mass (white arrows). (A) anillinHP/RS-derived embryo, late cellularization/early gastrulation. Cellularization is failing, but pole cell separation is almost normal. Boxed areas are shown at higher magnification in the insets. (B,b') Paired plasma membranes between two poles cells appear normal. (B,b'') Paired plasma membranes between two cellularized cells have completely vesiculated. anillinPE/PE derived embryo, late cellularization/early gastrulation. Cellularization is less perturbed than (C) in this weaker allele, but pole cell separation has failed completely. Boxed areas are shown at higher magnification in the insets. (c) No plasma membrane or vesicles are present between pole cell nuclei. (c') Partially vesiculated plasma membranes are present between the nuclei of cellularized cells. Scale bar: 2 µm in main panels, 0.5 µm in insets.





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