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Fig. 4. Hoxd1 mRNA can partially rescue the triple PG1 morpholino phenotype. Early tailbud stage embryos were injected on the left-hand side (lhs) with all three PG1 morpholinos (A1/B1/D1 MO) either alone (B,C), or in combination with Hoxd1 mRNA (E,F). Non-injected controls (NIC) and control morpholino (Cont MO) are also shown (A,D). Anterior is to the top. G: Krox-20 expression is partially rescued by Hoxd1 RNA. Numbers of embryos with two stripes (E), one broad stripe (F), one narrow stripe (B) or no stripes (C) of Krox20 expression were scored. Co-injection of Hoxd1 RNA partially rescued the Krox20 expression (n=29), which was either absent or very much reduced in embryos injected only with A1/B1/D1MO (n=18).





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