spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Knockdown of all three PG1 genes affects more posterior Hox genes. Non injected controls (NIC; A,C,E,G,I,K,M,O) and embryos injected on the left-hand side (lhs) with all three Hox PG1 morpholinos (A1/B1/D1MO; B,D,F,H,J,L,N,P) were analysed for Hox gene expression at early tailbud stage. Embryos are shown from the dorsal side, anterior to the top. Hoxb1 expression was lost (A,B: 70%, n=10), and Hoxa2 (C,D: 100%, n=18) and Hoxa3 (E,F: 100%, n=14) expression was almost completely absent. Hoxd3 (G,H: 68%, n=19) and Hoxd4 (I,J: 79%, n=14) neural tube expression was still present, but the pharyngeal arch expression was lost. The more posterior Hox genes (Hoxa5, K,L: 100%, n=10; Hoxc6, M,N: 71%, n=14; Hoxb9, O,P: 93%, n=14) had reduced neural tube expression in the anterior part of the embryo but their posterior expression domains (arrow) and mesodermal expression were unaffected.





Right arrow Return to article