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Fig. 2. Precursor proliferation in the brains of frizzled 9 mutants. (A) Phospho-Histone-H3 is a marker of M-Phase of the cell cycle. Shown are examples of staining from brains at E14.5, E18.5 and P8. (B) Semi-quantitative analysis of the numbers of M-Phase cells showing significant increases in the medial cortical wall of mutant mice at E14.5 and in the dentate gyrus of E18.5 mutants but not P8 or adults. (C) Similar analysis of the numbers of BrdU labeled nuclei after acute BrdU administration at E18.5. *P<0.05; **P<0.005.





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