spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Targeting strategy and confirmation of genotype. (A) The genomic organization of the Pkb{gamma} wild-type allele (top) was disrupted using a targeting vector with an IRES-lacZ-Neo-cassette (middle). Targeting of the wild-type allele leads to disruption of exon 4 of the Pkb{gamma} gene (bottom). Arrowheads indicate the localization of the primers for the PCR reaction. (B) The genotype of mice was determined by PCR. Representative results from Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice are shown. (C) The levels of PKB{gamma} in the brains of Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice were determined by western blot analysis using a PKB{gamma}-specific antibody. (D) The levels of PKB{alpha} and PKBß, respectively, were determined in brain lysates from Pkb{gamma}+/+, Pkb{gamma}+/- and Pkb{gamma}-/- mice using PKB{alpha}- and PKBß-specific antibodies.





Right arrow Return to article