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Fig. 2. Tissue distribution of PKB ${gamma}$ and levels of PKB ${alpha}$ and PKBß in Pkb ${gamma}$ mutant mice. (A) The mRNA level of PKB ${gamma}$ was determined in 15 different organs from adult Pkb ${gamma}$ ^+/+ mice. Total RNA was isolated from three adult mice and the levels were normalized to the level of PKB ${gamma}$ in the brain (100%). MG, mammary gland. Error bars represent s.d. mRNA levels of (B) PKB ${alpha}$ and (C) PKBß were determined using total RNA from six different organs of adult Pkb ${gamma}$ wild-type (n=3; black bars) and mutant mice (n=3; white bars). mRNA levels of PKB ${alpha}$ and PKBß were normalized to the level of PKB ${alpha}$ in wild-type brain (100%). Error bars represent s.d. (D) Western blot analysis of PKB ${alpha}$ , ß and ${gamma}$ within ten different brain regions using isoform specific antibodies.

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