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Fig. 4. Dispensable role of PKB
for body weight and glucose metabolism. (A)
Body weights of male Pkb
+/+ (white bars) and
Pkb
-/- (black bars) mice were measured at different
points in time (n=5-8 animals per genotype). Error bars represent
s.d. NB, newborn. (B) Blood glucose concentrations from random-fed (RF) and
overnight fasted (ONF) mice (n=8; 6 months old;
Pkb
+/+ white bars and
Pkb
-/- black bars). Error bars represent s.d. (C)
Insulin tolerance test. Animals (n=6; 6 months old;
Pkb
+/+ white triangles and
Pkb
-/- black circles) were fasted overnight and
insulin (1 U/kg) was administered by intraperitoneal injection. (C) Glucose
concentrations were determined at indicated time points from whole blood
collected from tail veins. Values were normalized to the starting glucose
concentration at the administration of insulin. Error bars represent s.d. (D)
Glucose tolerance test. Animals (n=6; 5-6 months old;
Pkb
+/+ white triangles and
Pkb
-/- black circles) were fasted overnight and
glucose (2 g/kg) was orally administered. Blood glucose concentrations were
sampled at the indicated times. Values were normalized to the starting glucose
concentration at the administration of glucose. Error bars represent s.d.