DEV01861 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. Retinal axons degenerate in breaking up (brek) mutants. Z-projections of confocal image stacks showing lateral views of the tectum in 5-6 dpf live Brn3c:mGFP larvae. At 5 dpf, retinal axons cover the tectum normally in brek mutants (C), similar to wild type (A). At 6 dpf, retinal axons become punctate in the brek tectum and coverage is more sparse (D) than in wild type (B). Arrowheads indicate puncta. Asterisks indicate melanophores in the skin. Scale bars: 20 mm.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. Retina defects in coming apart (coma) and beyond borders (beyo) mutants. Six dpf retina sections labeled with anti-GFP in green and zpr1 in red (A-C). (B) In coma, dendrite-bearing, GFP-positive neurons (arrowhead) are found at the margins of the inner nuclear layer. (C) In beyo, the edge of the inner plexiform layer ‘smiles’, i. e. it bends toward the outer retina (arrow).