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Fig. 6. Laminar specificity is perturbed in dragnet (drg)
mutants. (A-D) Analysis of retinorecipient layers in 6 dpf wild type (A,C) and
drg (B,D). (A,B) Z projections of confocal image stacks,
labeled with Brn3c:mGFP. Larvae are mounted at a slightly oblique
angle to better visualize the gap between SO and SFGS. (C,D) Optical sections
of Brn3c:mGFP tecta, stained with whole-mount anti-acetylated tubulin
(red) and anti-GFP (green). Arrowheads indicate ectoptic axon fascicles
traveling between SO and SFGS in drg mutants. (E,F) Analysis of the
retinotopic map in 6 dpf wild type (E) and drg (F). DiI (red) and DiO
(green) were pressure injected into ventronasal and dorsotemporal retina,
respectively (see inserts for illustration of injection sites). Axon targeting
in drg (F) is comparable with wild type (E), suggesting that
positional information along the retinotopic axes is intact. (G-N) Analysis of
the inner plexiform layer (IPL) in sections of 6 dpf retina (see M,N for
labels of retinal layers). (G,H) DAPI labeling. (I,J) Anti-GFP labeling to
visualize the four sublaminae to which Brn3c:mGFP RGC dendrites
project. (K,L) Anti-parvalbumin labeling, to highlight a subpopulation of
amacrine cells that project to three sublaminae in the IPL. (M,N)
Triple-labeling (merged image) showing DAPI (blue), anti-GFP (green) and
anti-parvalbumin (red). Formation of IPL sublaminae is not affected in
drg. An apparently unrelated phenotype of drg can be seen
using the DAPI stain: an abnormal aggregation of cells in front of the
drg lens (H). Asterisks indicate melanophores in the skin. Scale
bars: 20 µm in A,G.
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