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Fig. 6. Anteroposterior polarisation of the limb buds is affected in
Msx1-/-; Msx2-/- double mutant embryos after
11.5 dpc. (A,B) At 11.5 dpc, in normal embryos, Fgf4 is expressed in
the posterodistal part of the AER, and excluded from the anterior fourth of
the AER. In the double mutants, Fgf4 expression level is normal but
expression domain is shifted anteriorly (A,B, arrowheads). (C,D) At 12.5 dpc,
Fgf4 is still detectable at the anterior tip (white arrow in D) of
the AER in double mutant embryos. (E-H) Hoxd11 is expressed only in
the region corresponding to the future digits II to V in both normal hind- (E)
and forelimbs (G). In the double mutants (F,H), it is expressed along the
whole distal mesenchyme regardless of whether the limb bud (generally
hindlimbs) is truncated (F) or, conversely (essentially forelimbs), overgrown
anteriorly (H). (I,J) In a few double mutant embryos, Shh is faintly
expressed ectopically at the anterior margin of the forelimbs (J, arrow). The
posterior domain is slightly shifted distally (arrowheads). (A-D,G-J)
Forelimbs. (E,F) Hindlimbs. In all panels, anterior is towards the right.
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