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Fig. 2. Soluble signal from retinal cells inhibits postnatal proliferation. (A)
Experimental protocol (see text). Cells were cultured in the presence of 5
million retinal cells for 12 hours, BrdU was added for the final 2 hours of
progenitor cell culture (B,D) and the final 6 hours of glial cell culture
(C,E). (B-E) Example fields of nestin+ (red) BrdU-labeled (green)
progenitor cells (B,D), and CRALBP+ (red) BrdU-labeled (green)
Müller glia (C,E), under control conditions (no added cells: B,C) or with
the addition of 5 million dissociated retinal cells (D,E). (F) Quantification
of BrdU+/nestin+ cells shows a significant reduction in
proliferation with 5 million added cells (15±2.2%) compared with
control (24.9±2.4%). (G) Quantification of
BrdU+/CRALBP+ cells shows significant inhibition of
glial proliferation with addition of 5 million cells (23.0±2.8%)
compared with control treated cells (44.3±2.3%).
*P<0.02, pairwise comparison (Student's
t-test).
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