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Fig. 6. TGFß treatment reduces proliferation in P4 retina. P4 retinas were
cultured as whole explants for 24 hours with BrdU. (A,B) BrdU labeling of
control (A) and TGFß2-treated (B) explants. Fewer BrdU-positive cells are
present in TGFß2-treated P4 explants. (C) P4 explants were dissociated
after 24 hours of culture and processed for immunohistochemistry. TGFß1
(gray bar) and 2 (dark gray bar) reduced proliferation to 51.3% (±6.3)
and 52.4% (±1.2) of control (white bar), respectively. No significant
difference in BrdU+ cell numbers was observed in TGFß3 treated
explants (black bar). (D) Dose-response curve indicates 13.4% (±1.7) of
cells are BrdU-positive in control treated explants, compared to 8.4%
(±1.6) at 0.5 ng/ml TGFß2, 7.4% (±0.7) at 5 ng/ml, and 6.4%
(±0.4) at 50 ng/ml. *P<0.01,
**P<0.005, pairwise comparison (Student's
t-test).
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