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Fig. 8. Notch/Hes signaling pathway is activated in the add mutant retina.
(A) Lateral view of 34 hpf wild-type retinas expressing 
N-Tcf3. A
confocal image of a 4.5 µm plane is shown. Although ath5:GFP expression
(green) progresses to the large region of the neural retina in this stage (see
Fig. 1D), ath5:GFP spreads to
the dorsonasal retina in this plane. Some of N-Tcf3-expressing cells
(red) are ath5:GFP positive (arrows, yellow). (B) A confocal image of lateral
view of 34 hpf add mutant retinas expressing N-Tcf3. Very
faint ath5:GFP expression is detected in the ventronasal retina (green,
arrowhead). Cells expressing N-Tcf3 (red) in the dorsal retina do not
express ath5:GFP (arrows). (C-D) In situ hybridization of wild-type (C) and
add mutant (D) retinas with her4 RNA probe. In the wild-type
retina, her4 expression is localized in the central part of the CMZ
(lines and arrows), which corresponds to the intermediate zone between retinal
stem cells (asterisks) and differentiated neurons. her4 expression is
also observed in a part of the outer layer, where neurogenesis occurs at this
stage. By contrast, her4 expression remains in the large region of
the neural retina in the add mutant (D). (E,F) In situ hybridization
of wild-type (E) and wild-type retinas expressing NICD (F) with her4
RNA probe. her4 expression is upregulated in NICD-expressing retinas.
(G,H) In situ hybridization of mib (G) and add; mib double
mutant (H) retinas with her4 RNA probe. her4 expression is
not observed in either case. (I,J) In situ hybridization of wild-type (I) and
wild-type retinas expressing 47-ß-catenin (J) with her4
RNA probe. her4 is expressed in the CMZ and the presumptive outer
layer, the latter of which is undulated because of
47-ß-catenin-induced folding of the neural retina.
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